Brightase-TH

Tissue Dissociating enzyme for isolating primary cells

Brightase-TH is a recombinant Bacillus thermoproteolyticus rokko thermolysis that is expressed in Brevibacillus. In combination with Brightase-C, Brightase-TH provides a low endotoxin method to isolate primary cells.

No animal products are used in the production of Brightase-C.

NIPPI company name and logo is the property of NIPPI Inc., Japan.

Product Name

Brightase-TH Recombinant Thermolysin

Catalog Number

NP892-441: 4.7 mg

NP892-442: 2x4.7 mg (9.4 mg total)

Formulation

Lyophilized from 5  mL of a 0.94 mg/mL solution in 50 mM HEPES, pH 7.5, 2 M NaCl, 5 mM CaCl₂

Storage and Stability

Store at −20 °C soon after arrival. Thaw before use.

Stock Solution

Prepare a stock solution by adding 5 mL Sterile Water per vial and agitating gently at 4 °C. Divide stock solutions into single-use aliquots and store at −20 °C. Avoid repeated freezing and thawing.

Working solutions should contain ≥1 mM CaCl₂ for optimal activity and stability.

Quality Control

• Activity: ≥0.8 U/mg
• Endotoxin: ≤10 EU/mg

Notice To Purchaser

This product is for research use only, not for therapeutic or diagnostic purposes. It is not allowed to sell this product to a third party or use it for commercial purposes without permission from REPROCELL.

Datasheet

• Brightase-TH datasheet

1. Tanaka, K., Okitsu, T., Teramura, N. et al. Recombinant collagenase from Grimontia hollisae as a tissue dissociation enzyme for isolating primary cells. Scientific Reports volume 10, 3927 (2020). DOI: 10.1038/s41598-020-60802-z